Universität zu Köln 

Prof. Dr. Jane Parker

Max-Planck-Institut für Pflanzenzüchtungsforschung, Köln
email: parker@mpipz.mpg.de
phone: +49-(0)221 5062 303
website

Running time: 2003 – 2011

Abstract

Animal and plant intracellular immune receptors are assembled as constrained proteins that are selectively activated by pathogen effectors. We aim to define the activities of the RAR1 and SGT1 co-chaperones in the posttranslational control of the Arabidopsis immune receptors, RPP5 and RPS4. We will examine defects of rar1 and sgt1b mutants in the accumulation and interactions of different subcellular receptor pools, isolate a rar1 suppressor mutant (rsp1) that selectively recovers RPP5 resistance and explore the molecular basis of a newly discovered interaction between Arabidopsis SGT1 and cytosolic HSC70 chaperones.

(A final report will follow.)

Publications resulting from the project:

Shin, J., Heidrich, K., Sanchez-Villarreal, A., Parker, J.E. and Davis, S.J. (2012). TIME FOR COFFEE represses MYC2 protein accumulation to provide time-of-day regulation of jasmonate signaling. Plant Cell 24, 2470-2482.

Stuttmann, J., Hubberten, H.-M., Rietz, S., Kaur, J., Muskett, P, Guerois, R., Bednarek, P., Hoefgen, R. and Parker, J.E. (2011). Perturbation of Arabidopsis amino acid metabolism casues incompatibility with the adapted biotrophic pathogen Hyaloperonospora arabidopsidis. Plant Cell 23, 2788-2803.

Uppalapati, S.R., Ishiga, Y., Ryu, C.-M., Ishiga, T., Wang, K., Noël, L.D., Parker, J.E. and Mysore, K.S. (2011). SGT1 contributes to coronatine signaling and Pseudomonas syringae pv. tomato disease symptom development in tomato and Arabidopsis. New Phytol. 189, 83-93.

Stuttmann, J., Parker, J.E., and Noël, L.D. (2009). Novel aspects of COP9 signalosome functions revealed through analysis of hypomorphic csn mutants. Plant Signal. Behav. 4,1-3.

Stuttmann, J., Lechner, E., Guérois, R., Parker, J.E., Nussaume, L., Genschick, P., and Noël, L.D. (2009). COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis. J. Biol. Chem. 284, 7920-7930.

Llorente, F., Muskett, P., Sánchez-Vallet, A., López, G., Sánchez-Rodriguez, C., Ramos, B., Jordá, L., Parker, J., and Molina, A. (2008). Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi. Mol. Plant 1, 496-509.

Stuttmann, J., Parker, J.E., and Noël, L.D. (2008). Staying in the fold: The SGT1/chaperone machinery in maintenance and evolution of leucine-rich-repeat proteins. Plant Signal. Behav. 3, 283-285.

Zhou, F., Mosher, S., Tian, M., Sassi, G., Parker, J.E., and Klessig, D.F. (2008). The Arabidopsis gain-of-function mutant ssi4 requires RAR1 and SGT1b differentially for defense activation and morphological alterations. Mol. Plant-Microbe Interact. 21, 40-49.

Noël, L.D., Cagna, G., Stuttmann, J., Wirthmüller, L., Betsuyaku, S., Witte, C.-P., Bhat, R., Pochon, N., Colby, T., and Parker, J.E. (2007). Interaction between SGT1 and cytosolic HSC70 chaperones regulates Arabidopsis immune responses. Plant Cell 19, 4061-4076.

Kemmerling, B., Schwedt, A., Rodriguez, P., Mazzotta, S., Frank, M., Qamar, S.A., Mengiste, T., Betsuyaku, S., Parker, J.E., Müssig, C., Thomma, B.P.H.J., Albrecht, C., de Vries, S.C., Hirt, H. and Nürnberger, T. (2007). A brassinolide-independent role for the BRI1 associated receptor kinase (BAK1) in plant cell death control. Curr. Biol. 17, 1116-1122.

Azevedo, C., Betsuyaku, S., Peart, J., Takahashi, A., Noël, L., Sadanandom, A., Casais, C., *Parker, J. and *Shirasu, K. (2006). Role of SGT1 in Resistance protein accumulation in plant immunity. EMBO J. 25, 2007-2016. (* joint corresponding authors).